Reagents and solutions RIPA buffer: 20mM Tris (pH 7.5), 1mM Na2EDTA, 1mM EGTA, 2.5mM sodium pyrophosphate, 150mM NaCl, 1mM beta-glycerophosphate disodium salt hydrate, 1mM Na3VO4, 1µg/ml leupeptin hemisulfate salt, 1% sodium deoxycholate, 1% Triton X-100, 1mM PMSF Experimental procedure — Preparation of cell lysis solution 1 Cell collection: Choose logarithmic phase cells, wash cells with pre-cooled PBS, …
Primary cells refer to cells that are isolated or harvested directly from living tissue or organs. Primary cells closely mimic the physiological state of cells in vivo and are expected to maintain their in vivo functions under optimal conditions for a short period of time. The primary cells present several advantages over cell lines, including a …
Bridging the Gap in TKI-Resistant NSCLC: The Strategic Rise of Antibody-Drug Conjugates (ADCs) Introduction The treatment landscape for Non-Small Cell Lung Cancer (NSCLC) has been revolutionized by EGFR Tyrosine Kinase Inhibitors (TKIs). However, the inevitable development of acquired resistance limits their long-term efficacy. To bridge this therapeutic gap, Antibody-Drug Conjugates (ADCs) have emerged as a …